[Objective] The aim was to investigate the effect of four extraction meth- ods on extraction ratio of 11-α-Hydroxy-Canerenone from biotransformation broth. [Method] Previous reported method, soaking, elution method and homogenate meth- ods were used. [Result] Results showed that more than 90% of the biotransformed 11-α-Hydroxy-Canerenone existed in the broth supernatant and mycelia surface. Elu- tion and soaking methods were effective for 11-α-Hydroxy-Canerenone extraction. Extraction yields of 96.0% and 98.8% were obtained by eluting with 400 ml of ethy- lacetate and soaking with ethylacetate for 90 min, respectively. [Conelusion] The method is simple and efficient, showing industrial application potential.
[ Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction biotransformed by Hericium erinaceus. [ Method ] The free radical scavenging ability and reducing capacity of DPPH as well as inhibitory rate of nonenzymatic glycation reaction were measured targets for comparing changes of anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of fermentation lyophilizer and fermentation extraction before and after EGB fermention adsorbed by AB-8 macroporous resin. The EGB fermention was biotransformed by Hericium erinaceus. [ Result ] After adsorbed by AB-8 macroporous resin, the bioactive matters were concentrated and separated. The free radical scavenging rate, reducing capacity and inhibitory rate of nonenzymatic glycation reaction were increased significantly after adsorbed by AB-8 macroporous resin. [ Conclusion] AB-8 macroporous resin could be used for preliminary concentration of EGB fermentation which was biotransformed by Hericium erinaceus.
