利用抗坏血酸对AgNO3进行还原，生成银纳米粒核心，并通过正硅酸四乙酯的水解与聚合反应获得SiO2介孔外壳，制备平均粒径约为.9 nm的Ag SiO2核壳型纳米粒。 Ag SiO2纳米粒可以显著地抑制香石竹镰刀菌的生长，最小抑菌质量浓度为μg/mL，并可抑制香石竹镰刀菌菌丝生长和孢子分生。 Ag SiO2纳米粒处理～4 h后，菌丝体的过氧化氢酶、总超氧化物歧化酶、过氧化物酶活力增强，提示Ag SiO2纳米粒抗菌机制和活性氧诱导相关。
With Summer Resort,Clear Ripples Garden,Humble Administrator's Garden and Lingering Garden as the study subjects,the paper had focused on researches on water surface landscaping techniques and relevant theories of four famous gardens in China.Water bodies in Summer Resort aimed to manifest the sentimental appeal of lower reaches of Yangtze River,and water surface treatment techniques were based on local characteristics which were dense river network,sandbars and islands surrounded by water and scattered distribution of rivers.Sandbars,islands,banks and bridges were utilized to divide water surface,so as to display basic natural feature of water surface at lower reaches of Yangtze River.Water bodies in Clear Ripples Garden aimed to imitate the artistic concept of West Lake.As for its water surface treatment technique,divided "west dyke" and branch dykes had been used to separate water surface,so as to endow it with the ideological content of "fairy court" and "telepathy between human and nature".Water bodies in Humble Administrator's Garden and Lingering Garden aimed to realize "all rivers and lakes" with "one scoop of water",so that people could associate the artistic conception of a vase expanse of water.The study could provide reference for designers of landscape architecture to conduct water surface design.
With the pilings of rockeries,bridge trails,doors and windows,pavilions,towers,walls,halls and studios,corridors and courtyards as the research subject,illustrated with a large number of pictures,the relationship between architecture and rockery in classical gardens was elaborated.In classical gardens,their harmonious,complementary and positive aspect was emphasized,and their opposite,repulsive and passive aspect was restricted,and even the passiveness could be transferred into positivity,so as to make the beauty of architecture and the beauty of nature coexist,coming to the state that the artificiality and the nature blend into one whole.The research was to provide reference for the design and construction of modern gardens.
[Objective] This study aimed to investigate the structural character of xy- Ioglucan endotransglycosylase/hydrolase （XTH） gene in ethylene-insensitive feverfew. [Method] The total RNA was extracted from Chrysanthemum rnorifolium petal using Trizol reagent, and the cDNA fragment of XTH gene was cloned by RT-PCR and T/A cloning. [Result] The sequencing result showed that the cloned cDNA sequence was 911 bp. It was predicted to encode a polypeptide of 293 amino acids and had seven active sites of XTH family, and then named as CmXTH （gene accession number HM752243）. In addition, The BLAST analysis showed that the deduced amino acid sequence of CmXTH showed high homology with other 19 chosen plant XTHs. Among of these, CmXTH shared closer genetic relationship with Gerbera jamesonii, Solanum lycopersicum, whereas had relatively distant relationship with Populus euparatica, Fragaria ananassa, Actinidia deliciosa, etc. [Conclusion] The cloned fragment was certainly the cDNA of XTH gene, which was associated with the petal growth and senescence in Chrysanthemum morifolium.
