Background:Long non-coding RNAs(lncRNAs)plays an important role in the progression of gastric cancer(GC).Their involvement ranges from genetic regulation to cancer progression.However,the mechanistic roles of RP11-789C1.1 in GC are not fully understood.Methods:We identified the expression of lncRNA RP11-789C1.1 in GC tissues and cell lines by real-time fluorescent quantitative polymerase chain reaction.A series of functional experiments revealed the effect of RP11-789C1.1 on the proliferation of GC cells.In vivo experiments verified the effect of RP11-789C1.1 on the biological behavior of a GC cell line.RNA pull-down unveiled RP11-789C1.1 interacting proteins.Western blot analysis indicated the downstream pathway changes of RP11-789C1.1,and an oxaliplatin dosing experiment disclosed the influence of RP11-789C1.1 on the drug sensitivity of oxaliplatin.Results:Our results demonstrated that RP11-789C1.1 inhibited the proliferation of GC cells and promoted the apoptosis of GC cells.Mechanistically,RP11-789C1.1 inhibited checkpoint kinase 1(CHK1)phosphorylation by binding ataxiatelangiectasia mutated and Rad3 related(ATR),a serine/threonine-specific protein kinase,promoted GC apoptosis,and mediated oxaliplatin sensitivity.Conclusion:In general,we discovered a tumor suppressor molecule RP11-789C1.1 and confirmed its mechanism of action,providing a theoretical basis for targeted GC therapy.
Wenwei LiuWei FengYongxin ZhangTianxiang LeiXiaofeng WangTang QiaoZehong ChenWu Song
