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曹树威

作品数:6 被引量:10H指数:2
供职机构:海南大学农学院更多>>
发文基金:海南省教育厅高等学校科学研究项目博士科研启动基金国家自然科学基金更多>>
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香蕉纤维脱胶菌的筛选及其产酶条件的初步研究被引量:2
2011年
香蕉纤维是纺织行业的一种新型纤维原料,其脱胶方法和工艺研究的缺乏制约着相关的开发与利用。本文就香蕉纤维的微生物脱胶进行了初步研究,采用透明圈法从土壤中筛选到一株具有降解香蕉半纤维素能力的放线菌菌株51181,利用它脱胶可去除香蕉纤维38%的胶质;对菌株51181产半纤维素酶的条件优化后,该菌株所产的半纤维素酶活达48.63U/mL,表明该菌株在香蕉纤维微生物脱胶及酶法脱胶方面具有一定的应用前景。
黄小龙陶祥艳曹树威黄东益
关键词:香蕉纤维微生物脱胶半纤维素酶
Research on Flowering Habits and Fertility of Gaozhou Common Wild Rice (Oryza rufipogon) in Lingshui of Hainan Province被引量:1
2011年
[Objective] The paper was to explore the flowering habits and fertility of Gaozhou common wild rice (Oryza rufipogon Griff.) in Lingshui of Hainan Province. [Method] The flowering habits, pollen fertility and seed setting rate of Gaozhou common wild rice in Lingshui of Hainan Province were studied, and compared with the flowering habits of common wild rice in Hainan Province. [Result] Gaozhou common wild rice was able to flower twice a year. The first flowering stage was from mid-April to mid-June for about 60 d, and the daily peak flowering dates were from May 13 to May 22. The daily flowering time was mainly between 11:20 and 13:00, and the peak flowering was at 11:30. The second flowering stage was from late August to late October for about 45 d, and the peak flowering dates lasted from September 8 to September 18. The daily flowering time was mainly from 11:15 to 12:50, and the daily peak flowering was at 11:30. In both flowering stages, the panicles had the same flowering characteristics. The stigma exsertion reached a high value of 95.37% with single (46.23%) and dual (49.14%) stigmas exsertion respectively. A spikelet remained open for 1.5-2.5 h, the angle of spikelet opening varied from 29.9° to 32.4°. At the two flowering stage the average pollen fertility rates were 89.67% and 85.62%, the average seed setting rates were 58.81% and 55.79%, respectively. Hainan population flowered only once (from mid-October to mid-December), this had significant difference with the performance of Gaozhou common wild rice which flowered twice a year. [Conclusion] The results provided basis for the research on gene flow frequency from transgenic rice to wild rice and its relationship with flowering habits of common wild rice.
余昌花袁潜华徐立新何美丹李厚奇曹树威
高州普通野生稻在海南陵水的开花习性和育性研究被引量:2
2011年
[目的]探讨高州普通野生稻(Oryza rufipogon Griff.)在海南陵水的开花习性和育性。[方法]对广东高州普通野生稻在海南陵水的开花习性、花粉育性和结实率进行了研究,并与海南普通野生稻开花特性进行了比较。[结果]广东高州普通野生稻在陵水1年开花2次,第一开花期在4月中旬至6月中旬,历时约60d,开花高峰期为5月13~22日,日开花历时主要集中在11:20~13:00,日开花高峰在11:30左右;第二开花期在8月下旬至10月下旬,历时45d,开花高峰期在9月8~18日,日开花历时在11:15~12:50,日开花高峰在11:30左右。2次开花期间的颖花开放特性基本相同,柱头外露表现为单露率46.23%,双露率49.14%,柱头总外露率达95.37%。单个颖花从开颖到闭颖一般需1.5~2.5h,开颖角度为29.9°~32.4°。上、下半年2个季次的花粉育性分别为89.67%和85.62%,平均结实率分别为58.81%和55.79%。海南普通野生稻一般1年仅开花1次,开花期在10月中旬至12月中旬,这与高州野生稻在陵水1年开花2次的表现有明显差异。[结论]为研究转基因水稻向普通野生稻基因漂流频率及与普通野生稻的开花习性相互关系奠定了基础。
余昌花袁潜华徐立新何美丹李厚奇曹树威
关键词:高州普通野生稻开花习性花粉育性结实率
天然胶乳微生物凝固菌种的筛选鉴定及其凝固性能的研究被引量:1
2011年
用划线分离的方法从天然胶乳中分离出106株细菌,再从中初筛出16株产酸菌,并通过不同产酸菌对天然胶乳凝固效果复筛实验,最终筛选出3株凝固效果较好的菌种,分别命名为NRC1、NRC2与NRC3。实验结果表明:此3株菌都能有效缩短天然胶乳的凝固时间,并且在凝固过程中具有臭味小,发胀不明显等特点;单菌种微生物凝固NR和酸凝固NR的性能对比,具有快速塑性值较大,塑性保持率稍低,拉伸性能好等特点。测定3株细菌的16S rDNA序列,并对其进行分子鉴定。结果表明:NRC1与NRC3为克雷伯氏菌属(Klebsiella),NRC2为变形杆菌属(Proteus),均属于肠杆菌科。
王斌廖双泉马立胜李永杰曹树威
关键词:微生物凝固菌种鉴定
一株淮山炭疽病拮抗放线菌的分离及初步鉴定被引量:4
2011年
[目的]从海南药用植物根际分离得到对淮山炭疽病具有拮抗活性的放线菌菌株,并对其分类地位进行鉴定。[方法]采用HV培养基从盾叶鸡蛋花根际土壤分离放线菌菌株;通过平板对峙培养和发酵代谢产物抑菌试验筛选活性放线菌;根据形态特征,结合16SrRNA基因序列分析鉴定活性放线菌的分类地位。[结果]从盾叶鸡蛋花的根际土壤分离到12株放线菌,筛选到1株对淮山炭疽病菌具有良好拮抗活性的菌株51173,菌株51173鉴定为链霉菌属Streptomyces violaceusniger16S rRNA gene clade中的成员。[结论]放线菌菌株51173在淮山炭疽病菌的生物防治中将具有很好的开发应用前景。
黄小龙曹树威周双清黄东益汤维
关键词:炭疽病放线菌拮抗
Isolation of an Actinomycete Strain from Medicinal Plant Rhizosphere Soils with Inhibitory Activity against Yam Anthracnose(Colletotrichum gloeosporioides) and Its Preliminary Identification
2011年
[ Objective ] The paper was to isolate actinomycete strains with antagonistic activity against yam anthracnose from the rhizosphere soil of medicinal plants in Hainan Province, and identify its taxonomic status. [ Method ] Using HV medium, actinomycete strains were isolated from the rhizesphere soil of frangipani (Plumeria rubra L. ). The active antagonistic actinomycetes were selected by using confrontation culture method and antifimgal test of fermentation products. Ac- cording to morphology, combined with 16S rRNA gene analysis, the taxonomic status of active actinomycetes was identified. [ Result] Twelve strains of actinomyce- tes were isolated from the rhizosphere soil of medicinal plant frangipani. Strain 51173 showed significant antifungal activity against Colletotrichum gloeosporioides, which was identified to be a member of Streptomyces violaceusniger 16S rRNA gene clade. [ Conclusion] Strain 51173 showed a good application potential for bio- logical control against yam anthracnose.
黄小龙曹树威周双清黄东益汤维
关键词:ANTHRACNOSEACTINOMYCETES
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