The effects of acid hydrolysis pretreatment of corn cob on saccharification efficiency and fermentation bio-hydrogen production were investigated by orthogonal test design.The hydrolysis pretreatment of corn cob with dilute sulfuric acid was essential for adequately converting substrate into soluble saccharides and bio-hydrogen.The experimental results showed that acid hydrolysis pretreatment of corn cob was found most effective at hydrolysis temperature of 115℃,sulfuric acid concentration of 1.0%,hydrolysis time of 1.5 h and solid-liquid mass ratio 1∶10,in which both maximum saccharification efficiency of 0.5433 g·(g TVS)-1 and bio-hydrogen yield of 85.8 ml H2·(g TVS)-1 were observed.In addition,the mechanisms of degrading corn cob by acid hydrolysis and hydrogen fermentation were also discussed by means of X-ray diffraction(XRD)analysis and crystallinity calculation.
