Winter lignified stems of Bauhinia galpinii were used as explants for tissue culture,and the effects of different hormones on inductivity rate and rooting rate were investigated by supplementing different concentrations.The results indicated that buds were induced from a 2 cm high young plantlet cultured in MS medium supplemented with 1.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA for 20 d,and the induction rate was 100%.The mean amount of buds was 8.7.The medium of MS+ 0.4mg·L-1 NAA was used for rooting and the rooting rate was 85%.The experiment strategy to establish tissue culture system from lignified stem was feasible,which could be used for cold acclimation and cold resistant mutant selection.
