The research studied opportunities and potential threats confronted by agricultural sustainable development, laying foundation for optimization of agricultural structure, transformation of agricultural development mode, and accomplishment of agricultural modernization. On basis of development target, development theory and route of the 13^th Five-year Plan, the research analysis the opportunities and potential threats of Yunnan agriculture and proposed policy suggestions on sustainable development of agriculture from the perspectives of ecological and competitive advantage, as well as sustainable development division in order to provide references for scientific formulation and decision-making of relevant regional planning.