Triterpene acids components in leaves of Rosmarinus officinalis Linn.were separated and identified by HPLC,silica gel column chromatography and reversed-phase C18 semi-preparative liquid chromatography method.And taking the minimum inhibitory concentration(MIC)as the index,the inhibitory activity of each compound was determined.The results show that 4 triterpene acids compounds are isolated from ethanol extracts of leaves of R.officinalis;according to the peak characteristics of reference substances,compound 2,3 and 4 are identified as betulinic acid,oleanolic acid and ursolic acid,respectively;based on physicochemical constants and spectral data,compound 1 is identified asΔ^20(30)-ene-ursolic acid.Ursolic acid,Δ^20(30)-ene-ursolic acid and oleanolic acid have a certain inhibitory activity to Gram-positive strains(methicillin-resistant Staphylococcus aureus,MRSA),in which,the MIC value of ursolic acid to MRSA is 8.000-128.000μg·mL-1,and its inhibitory activity is the strongest;however,none of 4 compounds shows inhibitory activity to Gram-negative strain(Escherichia coli S002).
