[目的]探明Cry1Ab蛋白通过培养基-果蝇-拟环纹豹蛛这一食物链的传递,最终在拟环纹豹蛛（Pardosa pseudoannulata）体内的富集情况以及毒蛋白随食物链的传递在各营养级中的变化规律。[方法]采用酶联免疫技术（ELISA）测定Cry1Ab蛋白在拟环纹豹蛛和果蝇中的含量。[结果]Cry1Ab蛋白对不同龄期拟环纹豹蛛的体长、背甲宽、中眼域宽、腿长等生长指标不存在显著性差异,但对体重产生一定的影响,使得其体重略有下降的趋势。在0-10 d Cry1Ab蛋白在拟环纹豹蛛体内不断累积,但10 d后拟环纹豹蛛体内Cry1Ab蛋白含量逐渐降低。[结论]Cry1Ab蛋白在拟环纹豹蛛体内具有富集效应。
Objective] Field and laboratory observation was conducted to investigate Clubiona corrugate. [Method] The trials investigated the bio-ecology and behavior of the spider C. corrugate. [Result] The spider overwintered with spiderlings, adults and instars turn into adults after 6-8 molts. It had 2-3 generations each year in Hunan, and it owned the character of overlapping of generation in paddies. The average duration of generations of C. corrugate was 158.2 days, and the survival days av-eraged 223.2 days. The female and male mated several times without cannibalistic behavior, average number of eggs female laid throughout its adult life was 371.5, and hatchability can get 85.4%. The sex ratio was 1∶1. Its capability of resistance to starvation and drought was strong. Through indoor observation, the spider can sur-vive 25-61 days under the condition of no water and food. [Conclusion] The behav-ior of C. corrugate was also recorded in detail, and female usual y has a strong a-bility to protect their egg-sacs and spiderlings. At present, the bio-ecology and be-havior of the spider C. corrugate had not been reported.
