[Objective] This study aimed to explore the molecular mechanism of mulberry pigment metabolism regulation. [Method] Chalconesynthase（CHS） gene was cloned from Morus（Moraceae） in silico. The amino acid sequence, physical and chemical properties, transmembrane structural domain, hydrophobicity/hydrophilicity,subcellular localization, secondary and tertiary structure of protein were predicted and analyzed by bioinformatics tools. [Result] The cDNA sequence of CHS gene was 1 365bp by splicing using the software DNAstar and it contained a complete ORF including 1 170 bp which encoded 389 amino acids. Bioinformatic analysis showed that CHS gene included specific peptide sequence RLMMYQQGCFAGGTVLR of chalcone synthase superfamily, but has no signal peptide, belonging to the non-secretory proteins, located inside of cytoplasm. Its molecular evolution is more conservative.[Conclusion] The results above provided foundation for the further studies of structure and function of CHS protein.
