The mating behavior of cat flea, Ctenocephalides felis (Bouche) was studied on an artificial feeding device. Male and female can mate repeatedly with same partner or different ones. In the situation of male: female ratio of 1:5, each mating lasted an average of 6.6 min, with a mean interval between matings at 2.5 min., compared to 11.1 min and 12.1 min respectively in a cell with 5 males and 1 female. As many as 48 mating events were observed for one male during an 8 h period. One female mated 27 times in 7 h with 5 males in the same cell. Newly emerged males and females can not mate before blood meal and about 24 h blood feeding is required for successful mating. Newly emerged males can not mate with fed females (fed for 48 h), but fed males can mate with newly emerged females who are feeding the blood. Significantly more male contacts and male male mating attempts were observed after the paper treated with female extract was introduced into the cell. The paper contacts and mating attempts were 16.75-32.25 times and 15.75-31.38 times, respectively, on average during a period of 20 min when different doses (FE) of extract were provided.
用DNA条形码通用引物扩增了我国8种猛禽(14只个体)的线粒体细胞色素c氧化酶亚基I(CO I)基因,选取648 bp DNA条形码标准区域,结合99条猛禽条形码序列,探讨DNA条形码对猛禽的识别和鉴定。结果显示,75.6%的猛禽符合10×规则,且80.5%的猛禽均具有独特的DNA条形码可作为物种鉴定的依据,另有19.5%的猛禽(鵟属8种猛禽)由于种间差异太小,DNA条形码对它们的识别鉴定存在一定局限。
