[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, and Allium macrostemon. [Method] Herbal active ingredients were extracted by water boiling method and a bacteriostatic experiment was per- formed by oxford cup diffusion method, to select Chinese herbs with antibacterial effects. MIC values of seven kinds of Chinese herbal medicine were determined by in vitro half-fold dilution method. [Results] Root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, Allium macrostemon, Juncus effusus and Xanthi- um sibiricum had bacteriostatic effects on Escherichia coil and Staphylococcus au- reus in varying degrees. Xanthium and leaf of Ophiopogon japonicus performed bet- ter on Escherichia coil, with MIC values at 7.81 and 15.63 mg/ml, respectively. Panax pseudoginseng and Xanthium performed better on Staphylococcus aureus with MIC values at 15.63 and 31.25 mg/ml. And the drug＇s effect was positively correlated with the concentration. [Conclusion] Xanthium sibiricum is the best bacte- riostatic effect, followed by leaf of Ophiopogon japonicus, and Juncus effusus per- forms the poorest. Panax pseudoginseng showed the best bacteriostatic effect, fol- lowed by Xanthium sibiricum and Juncus effusus performs the poorest. These pro- vide references for clinic safety.
