Phytochemical investigation of Artemisia gmelinii Web. ex Stechm. led to the isolation of 11 known flavonoids. Their structures were identified as genkwanin（1）, hispidulin（2）, 3＇-hydroxy-genkwanin（3）, chrysoeriol（4）, apigenin（5）, 5,7,3＇,4＇-tetrahydroxy-6,5＇-dimethoxy-flavone（6）, kumatakenin（7）, quercetin（8）, patuletin（9）, quercetagetin-3,6,7-trimethylether（10） and 7,3＇,4＇-trihydroxy-3-methoxy-flavone（11） by spectroscopic methods. Ten compounds（2–11） were isolated from this title plant for the first time, and five compounds（3, 6, 7, 10 and 11） were isolated from genus Artemisia for the first time.
