应用质子核磁共振技术（1H-NMR）结合解卷积的峰提取方法,建立了中药黄芪注射液中氨基酸、有机酸类和糖类等8种初生代谢成分的含量检测方法。注射液样品经冷冻干燥后精密称取10 mg,加入0.6 m L D2O（含内标TSP 0.02 g/m L）用于NMR测试。根据1H-NMR指纹谱,从黄芪注射液中鉴定出20种初生和4种次生代谢成分。应用解卷积法提取乙酸、脯氨酸、焦谷氨酸、丙二酸、甘氨酸、葡萄糖、蔗糖、甲酸8种初生代谢成分的定量峰,然后进行峰面积积分,并采用内标法计算各个成分的含量。在给定浓度范围内,8种成分呈现良好的线性相关（R2〉0.9990）,精密度RSD〈3.8%,重现性RSD〈2.7%,稳定性RSD〈4.5%,回收率为91.4%~102.7%。结果表明,与常规处理方法相比,解卷积检峰再积分的方法可降低复杂体系中质子信号峰的重叠度,获得更加精确的定量峰积分面积,从而进一步提高1H-NMR含量分析的准确度。采用本方法对5个厂家共20批黄芪注射液样品中的8种初生代谢成分进行了含量测定,并运用主成分分析法对不同厂家的样品进行了聚类和区分。
The Rhodiola genus（Crassulaceae） is composed of about 90 species, mainly growing in high and cold regions. Some species are used as medicinal herbs to treat many diseases, such as cerebral hypoxia, cardiovascular disease, plateau response and so on. Up to now, about 180 constituents which are mainly glycosides, flavonoids, terpenoids, tannins, sterol and other compounds have been identified in the genus. The present review summarizes the chemical constituents isolated from the Rhodiola genus over the past few decades, with particular emphasis on Rhodiola crenulata（HK. f. et Thoms.） H. Ohba which is being studied by our research group.
