Production of transgenic mice with human decay accelerating factor (hDAF) gene will provide an effective way to study how to overcome xenogenic hyperacute organ. The expression vector of hDAF gene, PSP 64HD, has been constructed. The PSP 64 HD DNA were lineared and microinjected into the fertilized eggs of mice by using male pronucleus microinjection technique. The transgenic eggs were transplanted into the uterus mice. When newborn mice were 4 weeks old, their tail DNAs were isolated to do Dot and Southern blot analysis for their-self identification. The results show that the constructed foreign gene has been integrated into the mouse genome. It means that the expression vector of hDAF gene and its transgenic mice have been constructed successfully in our experiments. For the transgenic mice, 77.9% of injected eggs survived after microinjection, and 3.4% in pups after development. The integration rate for hDAF gene is 10.5%.
