The oxygen evolution, thermal dissipation, and photochemical energy storage of three hybrid poplar clones, namely the triploid clone B342, the diploid clone B11 [(Populus alba×P. glandulosa)×(P.tomentosa×P.bolleana)], and the triploid clone B346 [(P.tomentosa×P. bolleana)×(P. alba×P.glandulosa)], under light stress were studied using photoacoustics. The oxygen evolution signal and photochemical energy storage varied negatively with the pretreatment_PFD (photon flux density), whereas the thermal signal varied positively with the pretreatment_PFD. Photochemical energy storage was reallocated to PSⅡ more than to PSⅠ, while the photochemical energy storage in PSⅠ was more stable than that in PSⅡ when subjected to light stress. The inhibitors streptomycin (SM), dithiothreitol (DTT) and sodium fluoride (NaF) could all affect the oxygen evolution signal. Clones B11 and B342 were more resistant to light stress than clone B346.
There was heterogeneous distribution in stomatal conductance (g-s) and stomatal aperture for both high- and low-light leaves of Ligustrum sinense Lour. in four designated positions within a leaf. Linear or exponential or polynomial relationships between g-s and stomatal aperture were found when regression of g-s and stomatal aperture was established. Statistical analysis revealed that the relationship between g-s and stomatal aperture for high-light leaves was more significant than that of low-light leaves. A linear relationship between g-s and stomatal aperture existed in both positions 1 and 3 for both high- and low-light leaves. The stomatal density of the low-light leaves was much lower than that of the high-light leaves. The sensitivity of stomata to changing environment for high-light leaves was higher than that for low-light leaves, which may also relate to the higher stomatal density for the high-light leaves.
