By mRNA differential display from control versus NaCl_shocked Arabidopsis seedlings, we screened an Arabidopsis 3′ partial cDNA, which represents a gene encoding inositol 1,3,4_trisphosphate (Ins(1,3,4)P 3) 5/6_kinase_like protein. Northern blotting analysis showed that the gene, named as AtITL1, is strongly induced by NaCl and low temperature, but not induced by drought and abscisic acid (ABA). Analysis of 5′ region of the AtITL1 found that there are dehydration_responsive element/C_repeat (DRE/CRT) cis _acting elements, but no elements related to G_box and ABRE (ABA_responsive element) in its 5′ region, which is consistent with the expression patterns of the AtITL1 independent of ABA. These results suggest that the AtITL1 may be involved in the osmotic stress response pathway independent of ABA.
基于问题式学习(Problem Based Learning,PBL)是一种被广泛应用于医学教育的先进教学模式。在充分理解PBL教学理念的基础上,结合农业微生物实验课的特点,探讨了PBL教学法在农业微生物实验课中应用的可能性,并对其具体应用做出课程设计,以期将此教学模式引入农业微生物实验课,弥补传统教学体系中的一些不足,提高教学质量,培养高素质的创新型人才。
