For the first time, we have utilized high-performance liquid chromatography （HPLC） to simultaneously quantify the eugenol and bancroffione in Caryophylli Fructus. The optimized parameters included： Inertsil ODS-4 column （150 mm×4.6 mm, 5 μm）; column temperature： 35 ℃; mobile phase： methanol water （65：35, v/v）; flow rate： 1.0 mL/min; detection wavelength： 280 nm. Eugenol and bancroftione showed good linear relationships with peak areas within the range of （0.0998 0.8982） mg/mL （r = 0.9999） and （0.1474-1.3266） mg/mL （r = 0.9999）, respectively. The average recoveries were 102.52% and 100.96% for eugenol and bancroftione, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility to evaluate the quality of Caryophylli Fructus.
