[Objective]The research aimed study the amplification condition of SRAP-PCR of ginseng genome and set up the optimized amplification system/[Method]The effects of template DNA,primer,dNTP mixture and Mg^2＋ on PCR results were discussed.[Result] The optimized amplification procedure was as follows：pre-denaturing at 94 ℃ for 2 min;denaturing at 94 ℃ for 30 s,annealing at 48 ℃ for 30 s and extending at 72 ℃ for 1 min,40 cycles;extending at 72 ℃ for 7 min.The optimum reaction system was as follows：30 ng DNA template,2.0 μM upstream primer and downstream primer,0.3 mM dNTP mixture,2.5 Mm Mg2＋,the total volume was 25 μl.[Conclusion]The optimization amplification system for SRAP-PCR of ginseng was set up,which provided rapid and simplified test methods with good repeatability for SRAP analysis of the genetic relationship and genetic diversity of ginseng.
人参(Panax ginseng C. A. Meyer)是举世闻名的药用植物,我国是世界上人参生产大国与消费大国,高丽参在国际市场上以质量好负有盛名。我国人参产量约占世界总产量的一半以上,但人参产业产值不足韩国的1/4。本文分析中韩两国人参产业的现状,指出了我国人参产业的优势与不足,提出了我国人参产业的竞争策略。
