[Objective] The paper aimed to study effects of drought stress simulated by PEG on glucosinolates content in Arabidopsis thaliana.[Method] Drought stress was simulated by PEG-6000,ecological seeds of Arabidopsis thaliana were cultivated by the control group and drought treatment group respectively,Physical signs of Arabidopsis thaliana and contents of glucosinolates were determined after 0,4,5,6,7 d treatment.[Result] The results showed that leaf water content of rosette leaves was obviously decreased,leaf relative conductivity （characterized by membrane permeability） and the concentration of MDA increased,the extent of damage increased with the increased time.Content of total glucosinolate,aliphatic glucosinolate and indole glucosinolate increased got their maximum after 5 days treatment,and rapidly decreased after 6 and 7 days of treatment,even much lower than the control group.Each kind of glucosinolate changed with difference from each other.4MSOB which made the most proportion of the total glucosinolate changed consistently with the total glucosinolate and difference significant.As a whole,aliphatic glucosinolates were more sensitive to drought than indole glucosinolate.The proportion of some kind glucosinolate,like 4MSOB varied had correlation with the content change.[Conclusion] Drought stress had an effects on the contents of total glucosinolate,aliphatic glucosinolate and indole glucosinolate,which made the glucosinolate participated in defense response of plant to the outside of drought stress,but long-term drought stress in leaves was not conducive to the accumulation of glucosinolates.
Red Pine seedlings were grown in the potted soils with different acidity (pH=4.5,5.5,6.5,7.5,8.0) to investigate their physiological responses to soil pH,and futher to evaluate the soil pH feasibility. Dramatic physiological responses of Red Pine seedlings were detected under various soil pH treatments. Under soil pH 5.5,the seedling foliages grew best,with the lowest malondialdehyde content,ion leakage and soluble substance content,and the highest carotinoid content and antioxidant enzyme activities;With soil pH 7.5 and 8.0 treatments,the seedling needles developed poorest,with greater membrane lipid peroxidation and lower antioxidant enzyme activities,and the greater cell membrane injury and soluble substance content. Results suggested that Red Pine seedlings was mostly adapt to soil with pH of 5.5,followed by soils with pH of 4.5 and 6.5,and could hardly adapted to soil with pH of 7.5 and higher.
