Objective Aim to create a three-dimension（3D） reconstruction of acupuncture needling at Sidu（四渎 TE 9）.Methods First,the skin,subcutaneous tissue,muscles,the blood vessels and nerves near TE 9 were defined in anatomical terms.Second,the above anatomical structures including the dissected muscles associated with at TE 9,the underlying blood vessels and nerves were reconstructed in 3D.Third,the process of acupuncturing TE 9 on a virtual human body was simulated using the VOXEL-MAN technology.Results Both the local anatomy of the point and the process of acupuncture needling at TE 9 were reconstructed.Different layers of tissues were visualized during the process of needling,as the needle was inserted and manipulated at the point.Conclusions Virtual 3D reconstruction can contribute to research on the anatomical structure of acupoints and the visualization of the acupoints.We recommend that it be fully utilized in acupuncture research.
目的比较三种转染试剂对大鼠施万细胞(Schwann cell,SC)mi RNA的转染效率和细胞毒性,为更好地研究SC创造条件。方法以大鼠SC株(RSC96)为研究对象,采用Lipofectamine 2000、Fu GENE6 Transfection Reagent和Super Fectin II Reagent分别转染绿色荧光素FAM标记的mi RNA阴性对照产品,比较和分析三者的转染效率以找出转染效率最高的转染试剂,并以CCK-8法细胞活性检测和TUNEL凋亡染色对这三种转染试剂的细胞毒性进行观察。结果 Fu GENE6 Transfection Reagent不适合RSC96转染mi RNA,lipofectamine 2000转染效率偏低,Super Fectin II Reagent转染效率能够满足实验要求,但其细胞毒性较大,应注意换液。结论 Super Fectin II Reagent可用于RSC96转染mi RNA,转染后宜进行换液以减少毒性。
