试验筛选得到了一株产β-葡萄糖苷酶的枯草芽孢杆菌Z-1。利用七叶苷和柠檬酸高铁铵为底物,通过显色反应,在土壤中初筛得到15株产β-葡萄糖苷酶的菌株,复筛得到5株产酶活性较高的菌株,其中Z-1活性相对最高。对菌株进行生理生化和16S r DNA序列同源性比较分析,鉴定该菌株为枯草芽孢杆菌。采用DNS法对粗酶液的酶学特性进行了测定。
[Objective]The aim of this study was to screen the antagonistic spore-forming bacteria of Verticillium dahliae and identify its physiological and biochemical characteristics.[Method]Taking the cotton verticillium wilt pathogen Verticillium dahliae V-190 as the test microorganism,the antagonistic spore-forming bacteria were screened.Through the preliminary screening and secondary screening,an antagonistic bacteria strain 7-30 with stronger antibacterial activity was obtained,and its morphological characteristics,physiological and biochemical characteristics were also identified.[Result]84 antagonistic bacteria strains were isolated from soil in various places by the preliminary screening.Especially,18 strains with better antagonistic ability were screened again,so an antagonistic bacteria strain 7-30 with the diameter of inhibition zone 18.9 mm and stronger antibacterial activity was obtained.According to its morphological characteristics,physiological and biochemical characteristics,the antagonistic bacteria strain 7-30 was identified as Bacillus subtilis primarily.[Conclusion]The strain 7-30 was obtained as the antagonistic spore-forming bacteria of Verticillium dahliae.
