With Firmiana simplex （L.） W. F. Wight as an experiment material, the effects of different concentrations of GA3 （Gibberellin A3）, SA （Salicylic acid） and NAA （1-Naphthalene acetic acid） and stratification on the germination rate of seeds were studied firstly, and then the effects of GA3 and NAA on seedling growth were analyzed. The results showed that the germination rate could be improved by 0.075 g/L SA and stratification markedly. It was indicated by the experiment that the growth of the main root and main stem could be promoted by 0.050 g/L and 0.077 g/L NAA, respectively. However, the leaf length and leaf width decreased significantly in the GA3 and NAA treatments.
[Objective] This study was conducted to investigate the regulation of heat shock factor AtHsfA1a on ascorbate peroxidase under heat stress in Arabidopsis thaliana. [Method] After heat stress treatment on transgenetic A. thaliana with silenced endogenetic AtHsfA1a gene and wild A. thaliana plants as materials, the change in activity of APX enzyme was analyzed by spectrophotometry, the expression level of APX gene was investigated by real-time fluorescent quantitative PCR, and the binding condition of AtHsfAla with the promoter region of APX gene was analyzed by chromatin immunoprecipitation assay. [Result] The activity and mRNA level of APX in plants with silenced endogenetic AtHsfAla gene were higher than those in wild plants. Fragments of the promoter region of APX gene were not screened from the plants with silenced endogenetic AtHsfA1a gene, but found in wild plants. [Conclusion] This study provides a theoretical basis for the understanding of the important role of AtHsfAla in resistance to stress in plant, and is of great significance to the revealing of mechanism of resistance to stress in plant.
