Microarray chip including genechip has been of great application in fields of biology, quarantine, pharmacy and so on. Most of microarray chips were prepared by spotting aqueous droplet on the surface of substrate. Due to 'coffee-ring stain effect' , there always was a denser perimeter where sample was concentrated during the process of droplet drying after spotting. So the ununiformity of spot caused by 'coffee-ring stain effect' was one troublesome problem of the chip quality. Herein, superhydrophilic spot arrays on superhydrophobic AAO surface were fabricated to ease the 'coffee-ring stain effect' . Furthermore, a protein array is prepared on this patterned surface where immunoreactions are carried out. As a result, spot uniformity is greatly improved in a wide range of concentration and volume.
