[Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid (Lipaphis erysimi).[Method] Based on the morphological characteristics,the species of pathogenic fungus was identified.Spore shower method was used to carry out bioassay on the pathogen against turnip aphid.[Result] Primary conidia were ovoid,bitunicate and uninucleate,(24.7±1.4)μm×(10.7±0.9)μm,L/D=2.3±0.2.Secondary conidia had the similar shape with the primary ones,(18.6±2.1)μm×(13.3±1.3)μm,L/D=1.4±0.2.Hyphal body was like mycelium with the diameter of (10.6±0.8)μm.Conidiophores had palmate branch with the diameter of (10.0±0.9)μm.Pseudocystidia was not branched,which had rough base with the diameter of (19.2±1.7)μm,and gradually became more angular towards the apex with the diameter of (8.0±0.9)μm at tips.Rhizoid was like monohyphal shape with the diameter of (21.0±3.0)μm at base,the terminal apex had regular discoid holdfast.No resting spores were observed.The lethal dose of the pathogen against turnip aphid was 18.2/mm2.[Conclusion] The entomopathogenic fungus against turnip aphid was identified to be Pandora neoaphidis,and the pathogen was confirmed to have strong pathogenicity against turnip aphid.