The endosperm calli were induced on MS basic medium supplemented with lppm 2,4-D,0.5ppm KT and 5% sucrose.The medium which contained lppm BAP,0.1ppm NAA and 2%sucrose was used for cell suspension culture.In suspension cell culture,amitosis of cleavagedivision of nucleus have been observed after 5 days of culture.First the nuclear membraneand nucleolus disappeared.The crevice appeared in the center of the nucleus,and the nucleusdivided into two daughter nuclei of similar size and each with a nucleolus.The daughter nu-cleus resembled an eye in shape.FoIlowing;the emergence of cell wall,the two new unequalcells were produced.Such amitotic division proceeded repeatedly until the callus developedand eventually plantlet regenerated,
