Objective Nogo-A is an axon regeneration inhibitor, and its function in central nervous system （CNS） is still unknown. The present study is to explore the relationship between the expression of Nogo-A and the malignancy of oligodendroglial tumors in patients. Methods Tumor tissue samples with different malignancy grade were obtained from the hospitals. The samples used for detection had been diagnosed as oligodendroglial tumors （oligodendroglioma or anaplastic oligodendroglioma）. The expression of Nogo-A was detected by irmnunohistochemistry and western-blot analysis. The correlation test between the Nogo-A expression and the morphological changes （the percentages of atypical cells and mitotic cells in the tumors） related to the malignancy of tumor tissues was performed. Results There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligo- dendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group. Conclusion Nogo-A expression was negatively correlated with the malignancy grade of oligodendroglial tumors.
目的探讨在神经细胞早期生长发育过程中Nogo-A与Nogo-A受体(NG-R)的表达及意义。方法体外培养PC12细胞,实验组以神经生长因子(NGF)诱导其分化,对照组不进行诱导。倒置显微镜下选取20个随机视野进行观察,计数轴突生长和细胞增殖情况。采用免疫荧光染色、逆转录酶聚合酶链反应(RT-PCR)等方法检测PC12细胞生长及分化过程中Nogo-A与NG-R m RNA及蛋白的表达与变化。结果对照组PC12细胞未检出Nogo-A与NG-R的表达。在实验组NGF诱导PC12细胞向交感神经细胞分化过程中,Nogo-A与NG-R表达量逐渐增高(P<0.05)。结论在神经细胞发育早期,Nogo-A与NG-R可能发挥促进神经轴突生长的作用。
