The aluminum joints were prepared by overcasting liquid aluminum A356 onto 6101 aluminum extrusion bars. The microstructure, element distribution, hardness and tensile strength of the joint interface area were investigated, the mechanism of interface formation and fracture behavior were analyzed. The results show that good metallurgical bonding was formed in the joints by electro-plating the solid 6101 aluminum alloy with a layer of zinc coating and carefully controlling the overcasting process. There is a transition zone between the two bonded aluminum alloys, and the fine equiaxed grained structure in the transition zone is due to the high undercooling during solidification. The tensile strength of the joint interface is higher than that of the as-cast A356 aluminum alloy(about 145 MPa) and the final fracture is always located in the as-cast A356 material.
目的:探讨胃肠道弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)中之染色体t(14;18)(q32;q21)易位及Bcl-2基因扩增与DLBCL亚型分类及病人预后间的关系;探讨其在胃肠道DLBCL发病中的作用机制。方法:应用荧光原位杂交(fluorescence in situ hybridization,FISH)技术检测45例胃肠道DLBCL组织中之t(14;18)(q32;q21)染色体易位及Bcl-2基因扩增情况;应用免疫组化染色法检测该45例胃肠道DLBCL中之Bcl-2蛋白的表达。结果:在45例胃肠道DLBCL病人中,10例(22%)存在t(14;18)(q32;q21)易位,10例(22%)存在Bcl-2基因扩增。t(14;18)(q32;q21)易位阳性者与阴性者间的亚型分类比例差异有统计学意义(P<0.01)。Bcl-2基因扩增阳性者与阴性者间之生存时间差异有统计学意义(P<0.05)。而t(14;18)(q32;q21)染色体易位及Bcl-2基因扩增与Bcl-2蛋白表达间均属无关(P>0.05)。结论:t(14;18)(q32;q21)染色体易位与胃肠道DLBCL的免疫表型分类相关,生发中心B细胞(germinal center B cell-like,GCB)型与非GCB型间存在分子遗传学差异。而检测Bcl-2基因扩增对判断胃肠道DLBCL病人的预后具有重要意义。
目的探讨胃肠道弥漫性大B细胞淋巴瘤（diffuse large B—cell lymphoma，DLBCL）免疫表型与患者预后的关系。方法收集2000年6月至2007年6月我院普外科收治的胃肠道DLBCL63例，应用免疫组化方法检测抗体CDIO、Bcl-6和MUM1在胃肠道DLBCL中的表达，并根据检测结果进行分型。结果本组63例中CD10表达阳性13例，Bel-6阳性53例，MUM1阳性52例。根据检测结果将胃肠道DLBCL分为生发中心B细胞型（GCB型）17例（27％），非GCB型46例（73％）。GCB型局部淋巴结受累患者的比例（35％）显著低于非GCB型（70％）（P〈0．05）；肿瘤大小及浸润深度在两种亚型之间的差异无统计学意义。CD10表达阳性者的中位生存时间（76个月）显著长于阴性者（24个月）（P〈0．01）；GCB型胃肠道DLBCL患者的中位生存时间（76个月）显著长于非GCB型（28个月）（P〈0．05）；接受CHOP化疗方案的患者中GCB型的中位生存时间（76个月）显著长于非GCB型（24个月）（P〈0．05）；8例术后接受利妥昔单抗联合CHOP方案治疗的患者中GCB型和非GCB型各4例，目前均存活（22～47个月）。结论胃肠道DLBCL的免疫表型与局部淋巴结是否受累密切相关；CD10表达阳性者的生存时间显著长于阴性者；胃肠道DLBCL免疫表型分型对患者预后判断具有重要意义。
