为筛选出提取梨叶片总RNA的最佳方法,以梨极矮化突变体和苹果梨为材料,采用柱式植物RNAout试剂盒、SDS法和改良CTAB法提取其叶片总RNA并比较提取效果。结果表明,改良CTAB法能有效抑制酚类物质和多糖对总RNA提取的影响,获得纯度高、完整性好的总RNA,其28 S rRNA条带亮度高于18 S rRNA,所提取的RNA适合下一步的研究使用。
[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as materials for selecting the primary medium, subculture medium, rooting medium and acclimatization substrate during the tissue culture and rapid propagation. [Result] 6-BA was the optimal cytokinin for tissue culture of Lonicera edulis Turcz, compared with ZT; modified MS＋1.0 mg/L of 6-BA ＋ 0.2 mg/L of IBA was the optimal medium as primary and subculture medium, modified MS＋ 1.5 mg/L of IBA was the optimal medium for rooting of Lonicera edulis Turcz, the rooting rate had achieved 100% after cultured for 30 d. The optimal substrate for transplanting plantlets of Lonicera edulis Turcz was composed of humus and perlite （1∶ 1, V/V）, survival rate was as high as 95% after 30 d. [Conclusion] This study provided basis for the rapid propagation of superior seedlings of Lonicera edulis Turcz, as well as the establishment of industrialized breeding technical system and the implementation of scale production.
