Camptotheca acuminata Decaisne is a tree unique to China, producing terpenoid indole alkaloid (TIA), camptothecin (CPT), and 10-hydroxycamptothecin (HCPT), which possesses strong anti-cancer bioactivity. The dynamics of HCPT and CPT during seed maturation, seed germination, postgerminative development and daily growth were measured by High Performance Liquid Chromatography (HPLC). This article provides some evidence for presenting the theory that each characteristic of HCPT and CPT accumulation is under developmental regulation and then attempt to elucidate the metabolic relationships between them. HCPT accumulates particularly in young tissues and organs and is relatively unstable, while the distribution of CPT is more ubiquitous and stable. Their different distribution properties suggest that HCPT and CPT are differentially regulated and play distinct roles:during development.
Supercritical CO 2 extraction was applied to the extraction of flavonoids from licorice. The optimized conditions are: CO 2 pressure 30 MPa, temperature 50 ℃, solid to entrainer ratio 1∶5(g/mL), CO 2 flow rate 10 kg/h, separation pressure 5.5 MPa temperature 40 ℃. Results showed that the flavonoids yield obtained by supercritical CO 2 extraction was 2.2 times that obtained by common solvent extraction method.
设计了室内控制条件下的东北红豆杉和无花果的复合种植实验,并与单一种植东北红豆杉和单一种植无花果的模式进行了比较。分别考察了上述3种模式下,东北红豆杉和无花果的生长指标、光合作用以及土壤酶活性差异。结果表明:①在生长量方面,复合种植3个月的东北红豆杉的株高增长率为152.6%,是单一种植的东北红豆杉株高增长率的1.56倍;复合种植3个月的无花果,其当年生枝的生长量为68.4 cm 3,比单一种植的无花果高36.3%。②在光合作用指标方面,在800μmol·m-2·s-1光照强度下,复合种植3个月的东北红豆杉和无花果净光合速率与相应的单一种植相比,分别提高了19.0%和5.3%。③在土壤酶活性方面,在7月份,复合种植模式下的土壤蔗糖酶、脲酶和酸性磷酸酶活性比单一种植东北红豆杉土壤相应酶活性分别提高51.6%、58.5%和50.8%,比单一种植物无花果土壤相应酶活性分别提高85.5%、47.5%和71.9%;土壤多酚氧化酶活性在不同种植模式之间无显著差异(P>0.01)。[JP2]综上,东北红豆杉—无花果复合种植可以提高土壤蔗糖酶、脲酶和酸性磷酸酶活性,对两种植物生长均具有促进作用。上述结果可为在生产实践上进行东北红豆杉—无花果复合经营提供参考。[JP]
Camptothecine （CPT） and hydroxycamptothecin （HCPT）, two kinds of anti-cancer alkaloids, were extracted from Camptotheca acuminata leaves using homogenate extraction technology under different conditions such as the ratio of material to liquid, ethanol concentration, and homogenate time. The optimum technology parameters for homogenate extraction of CPT and HCPT from C acuminata leaves were determined as homogenate time at 8 rain, ethanol concentration at 55% and the ratio of material to liquid at 1：15 （g：mL）. By using the optimized parameters, we obtained 0.639‰ extraction rate for CPT and 0.437‰ for HCPT. The extraction yields of CPT and HCPT extracted by homogenating technology were higher than those by other extractive methods, such as ultrasonic, reflux, shaking in water bath. It is concluded that the homogenate extraction technology was an efficient method for extracting CPT and HCPT from C acuminata leaves, with characteristics of less extraction time and high yield.
