Sp-Octyl-(8-Chloroadenosine)-3’,5’-Cyclophosphate (OCC), a newlysynthesized cAMP analog, strongly induces growth inhibition and differentiation in human promyelocytic leukemia HL-60 cells. The effect of OCC is dose-time dependent. By using the LKB2277 Bioactivity Monitor,we determined the heat production rate of HL-60 cells treated by OCC at different concentrations. It was found that the heat production rate of HL-60 cell treated by OCC gradually decreased to that of normal human neutrophiles.
The heat production of Wistar rat polymorphonuclear leukocytes (PMN) was measured by an LKB 2277 Thermal Activity Monitor. When PMN were activited with phorbol-12-myristate13-acetate (PMA), the respiratory burst was recorded by greatly incr eased heat production. Experiment was also carred out in the present of the inhibitor, Total Flavonoids of Lycium Barbarum L. (TFL). The respiratory burst heat production peak was disappeared, but the heat production curve was higher than that of PMA because TFL increased the metabolic activities of PMN.
