Based on soil monitoring data in nine sites of Jinhu, Xuyi and Hongze counties on the south bank of Hongze Lake from 1982 to 2013, changes in soil p H, total nitrogen(TN) content, available phosphorus content, organic matter(OM)content and cation exchange capacity(CEC) were analyzed. The results show that due to excessive application of chemical fertilizer in soil on the south bank of Hongze Lake, soil p H reduced by about 2 on average, while TN content and available phosphorus content in soil increased by more than one time and 2-5 times respectively. Soil acidification caused by agricultural production was very serious. In addition, low soil p H resulted in serious loss of soil cation, so that soil CEC in2013 accounted for less than 50% of that in 1982 and affected mineral nutrient metabolism of crops. Therefore, application of calcium, potassium and trace-element fertilizer should be paid more attention to during agricultural production in future.