Aim To establish an RP-HPLC method for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract. Methods Chromatography was carfled out on Kromasil C18 column （250 mm × 4.6 mm, 5 μm）, using THF-CH3CN-H2O-H3PO4 （30 ： 5： 125 ： 0. 1） as the mobile phase at a flow rate of 1.0 mL·min^-1. The UV detection wavelength was 270 nm. Results The linear range of 2＂-O-rhamnosyl vitexin and vitexin were 0. 106 4 μg - 2. 1280 μg （ r =0. 999 1 ） and 0. 139 2μg - 2. 784 0 μg （ r =0. 999 3 ）, respectively. The average recoveries of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf were 99.2% （ RSD = 2.80%, n = 6） and 100.6% （ RSD = 2.84%, n = 6）, respectively. Conclusion This method is reproducible, simple, precise, and rapid for simultaneous determination of 2＂-O-rhamnosyl vitexin and vitexin in Chinese hawthorn leaf and its extract, thereby providinge the basis for quality specification of Chinese hawthorn leaf and its extract.
