By using the domestic silkworm as the bioreactor (reorganizing the nucleus polygon virus expression system) to produce genetic products, we can attain our goal with less cost, higher productivity and activity in a safe manner. With the swelling development of biosciences and biotechnologies in recent years, we should develop new experimental species, especially the lower species, to replace the mammals and explore the animals’ potential as a resource to benefit the human beings. We will make a detailed account of what we have achieved by utilizing the domestic silkworm exotic genes and the progress we have made in its animalization breeding technologies. Our domestic silkworm expression system is composed of its cells, transferring carrier, modified BmNPV, its chrysalis. We have obtained the rsj14NPV, which contains Japanese schistosome fatty acid albumen genes, and rsj28NPV, which contains Japanese schistosome 8KDGST genes. After being infected by the rsj14NPV, every silkworm (871×872) can produce 3.5 mgs’ purified albumen. After the silkworm 57A.57B×24.26 is infected with Rsj28NPV, every silkworm will turn out 5mgs’ purified albumen. By employing rSj14NPV(871×872),we observe the relations between the volume of contracted virus, the timing of the contraction, the duration of the virus and the amount of the produced albumen. The results are as follows: the silkworm that is inoculated with 4μL and 6μL produces 4-5mgs/silkworm, which is the highest, while the silkworm that is inoculated with 1μL group and 2μL group turns out only 1 mg, which is the lowest; the timing of the inoculation has no significant influence on the productivity; the reorganized virus that is preserved for a long period of time(9 months)is not very productive after being inoculated, which shows that we should use the required resurrected reorganized virus to achieve high productivity; by inoculating 55μLs’ resurrected reorganized virus(rsj14NPV) into the silkworm 871×872 will produce 4-5 mgs’ albumen, which is similar to tha
The expressed sequence tag of eukaryotic translation initiation factor 5 （eIF5） from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioinformatics method. The overlapping sequences were assembled into one that includes the complete open reading frame （GenBank accession number： AY686501）. The full-length cDNA of SjeIF5 was cloned into a pET-28c^（＋） vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coll. The recombinant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.
