[Objective] This study was conducted to investigate effects of application amounts of N, P and K and their ratio on tea quality components in mulberry leaves. [Method] Effects of sole application and combined application of N, P and K on the yield and tea quality components of spring mulberry leaves were studied by 3-factor 3-level experiment, based on previous experiments. [Result] Combined ap- plication of 3 of N, P and K was better than sole application and combined appli- cation of 2 from them. Insufficient or excessive fertilization both significantly reduced yield of mulberry leaves and contents of tea quality components, such as 1-de- oxynojimycin (DNJ), flavonoids, tea polyphenols and amino acids. With the yield of spring mulberry leaves as a target, it is advised to apply N, P and K at rates of 240, 150 and 60 kg/hm^2 , respectively. [Conclusion] This study provides reference and theoretical basis for the establishment of raw material base of high-quality mul- berry tea.