inducing resuscitation with herbal aromatics is important to modulate the brain intake of drugs in traditional Chinese medicine,but limited information has been available on the mechanism of action.The MDCK-MDRl monolayer is an excellent in vitro cell model to use as a tool to study blood brain barrier（BBB） screening.In this study,we established MDCK-MDR1 cell line by stable transfection and investigated the effects of several important herbal aromatics on BBB permeability.The characterization experiment demonstrated the MDCK-MDRl used in this study was valid.In a transport study,we found several herbal aromatics increased the permeability of fluorescein isothiocyanate-labeled dextran 4kDa（FD4） and inhibited efflux of Rhodaminel23（Rhol23）.These results demonstrated that herbal aromatics enhanced the BBB permeation of drugs by both inhibition of P-gp and opening of the BBB tight junction,thus providing new insights for understanding the mechanisms of aromatic compounds＇ BBB permeability.
