[Objective] The paper was to study the effect of agricultural land reform on cotton cultivation in Manasi, XinJiang based on reformed farmers. [Method] Rural land contract management rights transfer of rural household in Manasi County of Xinjiang Changji Hui Autonomous Prefecture were surveyed in August, 2011. Based on the survey data, the paper analyzed the effect of agricultural land reform on cotton culti- vation from 2 aspects of cotton cultivation scale and benefit, and also studied the willingness of farmers for agricultural land reform. [Result] The main obstacle for farmer's unwillingness of continuous land reform for cotton cultivation was that the cotton cultivation risk was big, and the benefit could not be guaranteed. The agricul- tural land area per household increased after agricultural land reform, which increased the modernization progress of cotton cultivation. It also increased the benefit of cotton cultivation, and further affected the benefit of cotton cultivation. [Conclusion] The study provided reference for improving scale cotton cultivation efficacy in Xinjiang.