触压觉和痛觉对于生物体感知周围环境有着重要的作用,这些行为的生物学基础依赖于将机械力转化为电信号的离子通道—机械敏感离子通道(mechanosensitive ion channels,MSCs)。MSCs同时参与其他生理功能,包括听觉、渴觉、动脉血管压力感觉等。以往研究发现,MSCs也在触觉、听觉、本体感觉中发挥关键作用。Beaulieu-Laroche等最近发现一种新型机械敏感离子通道TACAN(在波斯语中是“移动”的意思),又被称作为跨膜蛋白120A(Tmem120A)、NET29或者TMPIT,在痛觉感知中起重要作用。方法:①利用质谱技术分析筛选出可能的MSCs。
Objective The present study aimed to investigate the electrophysiological properties of wide dynamic range （WDR） neurons in spinal dorsal horn of rats with neuropathic pain induced by lumber 5 （L5） spinal nerve ligation （SNL） in a large size of samples.Methods Adult Sprague-Dawley rats were divided into normal and SNL groups.Electrophysiological technique was used to record the characteristics of WDR neurons in the spinal dorsal horn.Results Compared with the WDR neurons in normal rats,the WDR neurons in SNL rats showed an increase in excitability,manifested by an enlargement of the receptive field size,an increase in the proportion of neurons that exhibited spontaneous activities,decreases in the Cresponse threshold and latency,and an increase in the C-response duration.In addition,the numbers of A-and C-fiberevoked discharges were smaller in SNL rats than in normal rats.Conclusion The excitability of spinal WDR neurons increased in rats with neuropathic pain induced by L5 SNL.The increase in excitability of WDR neurons may contribute to the development of neuropathic pain.
