本实验利用化学诱变剂甲基磺酸乙酯(EMS)处理吸涨的沙枣种子,探讨获得沙枣变异植株的适宜诱变条件.实验设置4个EMS浓度(CK、0.5%、1%、1.5%、2%)和4个诱导时间(2、4、8、12 h),以致死量达到50%为标准.结果表明,EMS诱变抑制了沙枣种子胚的生长,生成的沙枣幼苗干鲜重降低,幼苗根长显著缩短.随着诱变剂量的增大和处理时间的延长,沙枣幼苗的成活率均呈现出显著下降的趋势.以幼苗成活率作为筛选指标,筛选出处理吸涨沙枣种子的最佳EMS诱变条件:EMS浓度为1%,处理时间约为3 h.
[Objective] The paper aims to provide references to cultivate I.galbana in a large scale and explore its medical value.[Method] I.galbana had been cultivated in different light-path flat plate bioreactors,and the growth condition and content of organic matter within cells of I.galbana had been analyzed.[Result] Growth rate and volumetric productivity of I.galbana cells increased as light-path of flat plate bioreactors decreased,however,daily areal output rate rose with the increasing of light path.The smaller the light path of flat plate bioreactors was,the more content of total lipids,protein and polysaccharide it had in I.galbana cells.[Conclusion] It is an effective way to improve production efficiently and reduce the cost by selecting the suitable bioreactors.
