Genetic map of sorghumsudan grass(314AZKSD) were constructed based on AFLP and RAPD markers with F2∶3 populations. The field experiments were designed with randomized blocks of 3 replications in 2 areas and involved 248 families of F2∶3. Ten main agronomic characters (including yield) were tested. The quantitative trait loci (QTL) and gene effects of 10 characters were analyzed with composite interval mapping (CIM) based on the genetic sergeants analysis. The main results were as follows:1. The linkage map of sorghumsudan grass of 158 AFLP markers and 8 RAPD markers was produced. It covered 836 cm of the genome. The average space between markers was 5.03 cm.2. From the 166 loci (158 loci based on AFLP markers and 8 loci based on RAPD markers), 136 loci significantly expressed the segregation ratio of 3∶1 through X-square test. The fitness was 81.9%, and the deviation rate 18.1%.3. Of the 10 agronomic characters, 48 QTLs were determined. Among them, 5 QTLs related to the plant height, 5 QTLs to the tiller number, 5 QTLs to the stem diameter, 4 QTLs related to leaf number, 6 QTLs to leaf length, 5 QTLs to leaf width, 4 QTLs to the length of a spike, 5 QTLs to fresh weight of the individual, 5 QTLs to dry weight of the individual, and 4 QTLs to the weight ratio of stem/leaf. The loci were located on 10 linkage populations separately.4. From the 98 QTLs detected in the materials of 2 different areas (48 QTLs from one, and 50 QTLs from the other), 6.1% QTLs (or 6 loci) expressed additive effect; 36.8% QTLs (or 36 loci) showed partly dominance effect; 17.3% QTLs (or 17 loci), dominance effect; and 39.8% QTLs (or 39 loci), super dominance effect. The super dominance effect and the dominance effect fulfilled the main function in the genetic basis of heterosis of sorghumsudan grass.5. Estimation of genetic parameters and correlation of 10 agronomic characters proved that the differences among the F2∶3 families were extremely significant. The correlations among most character