Taking the Technical Specifications of Eco-environmental Quality Assessment enacted by the State Environmental Protection Administration in 2006 as the conceptual framework model and improving some indices and weights in the regulation,the eco-environmental assessment index system for Manas River Basin was established,and the eco-environmental quality index (EQI) of this basin from 1976 to 2005 was assessed based on the related data.The results indicated that the ecological quality index increased to 48.26 from 34.44 during 1976-2005.The biologic abundance index,vegetation coverage index and land deterioration index decreased,while there was an increase in water reticulation density index,environmental quality index and pollution load index,so the improvement and deterioration of the ecological environment coexisted in the basin.On the whole,the poor state of the basin ecological environment had turned into a general state,and the eco-environmental quality was developed towards favorable circle.The results also showed that the improved calculating method of EQI accorded with the actual situation of the research area.