The FLS900 steady-state fluorescence spectrometer is used to test the blood in vitro radiated by the continuous laser. Variation components in the blood and the fluorescence characteristic decay with the time are given. Results show that when the blood is excited by the laser of 407 nm, the location of the fluorescence peak is unchanged, and the intensity of the emission peak at 605 nm is slowly decreased. But the fluorescence spectrum wave changes after 14 h, and eventually has a strong fluorescence peak at 474 nm. It is shown that the laser can obviously put off the decay time of the blood in vitro. After the laser continuously irradiates for a period of time, it can lead to the changes of cell morphology and accelerates the hemolytic process. The blood cell decay produces many fluorescent substances-the reduction of the nicotinamide adenine dinucleotide. The result provides a valuable reference to study the activity of blood cells and the functional abnormalities of the blood.
The fluorescence spectrum of the ether-water solution excited by the ultraviolet light with the wavelength of 245 nm is experimentally detected. Based on the second derivative analysis, the fluorescence spectrum of the ether-water solution is used as Gaussian decomposition and seven Gaussian spectral lines are obtained. The center wavelength, the peak intensity and the half peak bandwidth of each Gaussian spectral line are measured, and the multi-peak fitting is made by using Gaussian primitive parameters. The highest and the lowest oscillation energy level differences in the ground state of each Gaussian spectrum are calculated. It is found that there are seven types of luminescent association molecules formed by ether and water molecules in different configurations existed in the solution. The location of each optimum absorption wavelength and the half peak bandwidth of the Gaussian spectral line is different. The energy level difference with the central wavelength of 304 nm attains the maximum value The result can contribute to the study of the molecular association in ether-water solution.
