Objective To observe blood sugar values were measured at respectively, in 40 patients with type lI instant effect of acupuncture on lowering blood glucose. Methods Fasting 7:30 and 8:00 a.m. on one day, two and three days before acupuncture, diabetes. Acupuncture was applied at Yishu (胰俞 Extra), Feishu (肺俞BL 13), Pisha which was (脾俞 BL 20), Shenshu (肾俞 BL 23), Sanyinjiao (三阴交 SP 6), Taxixi (太溪 KI 3) and auxiliary points, followed by needle remaining for 30 minutes, for continuous 3 days. Blood glucose values measured immediately after acupuncture were compared with those obtained before acupuncture. Results Before acupuncture application, no significant differences were found between the values measured at 7:30 and 8:00 a.m. (P〉0.05) in fasting blood glucose values. Fasting blood glucose values measured at 8:00 were lower than those at 7:30 on the three days during acupuncture treatment (P〈O.05), and they were also lower than those measured at 8:00 before acupuncture (P〈0.05). Conclusion Acupuncture has immediate effect of lowering blood sugar.