[Objective] This study was to evaluate the ecological suitability of agricultural land in western Jilin Province, with the aim to provide basis for the effective application of agricultural resources and the structural adjustment of land use. [Method] The evaluation index system was constructed based on fuzzy mathematic method according to the principles of systematic, dominant, effectiveness and feasibility; based on law of tolerance, reasonable evaluation criteria were determined according to the ecological amplitude of crops; based on GIS processing, the initial data completed the mathematical operation by using the VBA program in Excel. [Result] The area of agricultural land grade I was 5 512 km2, grade II of 25 985 km2, grade Ill of 7 907 km2, and area of land not suitable for agriculture was 6 312 km2. According to the evaluation results, the key areas for land use adjustment were Zhenlai County, Da'an County, Tongyu County and western Changling County. The directions of land use adjustment included improving irrigation and drainage conditions, governing land salinization and conversion of cropland to forest and grassland. [Conclusion] This study provided basis for the effective application of agricultural resources and ecological environment construction in western Jilin Province.