It has been debated whether preference for subject-extracted relative clauses in language processing is a universal rule, with evidence from both first and second language acquisition studies. But very few studies focus on learners of Chinese as a second language. The current research studied Chinese subject/object-extracted relative clauses processing among the learners of Chinese as a second language by a self-paced reading experiment. The results demonstrate a faster and more accurate processing of subject-extracted relative clauses in both subject and object modifying conditions, adding more evidence to the universal preference for the subject-extracted relative clauses. Both Frequency-Based Accounts and Memory-Based Accounts are discussed related to the current findings.