触压觉和痛觉对于生物体感知周围环境有着重要的作用,这些行为的生物学基础依赖于将机械力转化为电信号的离子通道—机械敏感离子通道(mechanosensitive ion channels,MSCs)。MSCs同时参与其他生理功能,包括听觉、渴觉、动脉血管压力感觉等。以往研究发现,MSCs也在触觉、听觉、本体感觉中发挥关键作用。Beaulieu-Laroche等最近发现一种新型机械敏感离子通道TACAN(在波斯语中是“移动”的意思),又被称作为跨膜蛋白120A(Tmem120A)、NET29或者TMPIT,在痛觉感知中起重要作用。方法:①利用质谱技术分析筛选出可能的MSCs。
We studied the protective effect of xylocoside G on Aβ25-35-induced apoptosis in PC12 cells. Cell viability was analyzed by MTT assay, and apoptotic neuronal death was assessed by Hoechst 33342 staining. Flow cytometry was used to determine the apoptotie neuronal cells quantitatively. The level of intracellular reactive oxygen species （ROS） was monitored with the fluorescent probe 2＇,7＇-dichlorofluoresce in diacetate （DCFH-DA）. We found that PC12 cells treated with 25 pM Aβ25-35 for 24 h had a significant decrease in cell viability compared with control, and the percentage of apoptotic cells was increased to 34.26%. The level of intracellular oxygen species was also increased. Co-incubation with xylocoside G （10μM） for 24 h attenuated the damaging effect of Aβ25-5 on the cell viability, and the percentage of apoptotic cells was decreased to 22.62%. Moreover, the increase of ROS induced by Aβ25-5 was inhibited by xylocoside G （10μM）. We concluded that xylocoside G had protective effect against Aβ25-35-induced apoptosis, which might be related with the decrease of the level of ROS.
