With the development and decreasing cost of sequencing techniques, it is possible for scientists to conduct deeper research in phylogenomics. During the procedure of phylogenomic analysis, the mostimportant and vitalest step is orthology prediction, for that the prerequisite to phylogenetic reconstruction is that the genes being compared are orthologous. Here we briefly review the related concept of orthology anddifferent methods for orthology prediction. We also provide recommendations to give some advice for better selection of orthology prediction methods.
