Objective： To observe the therapeutic effect and safety of Qianggan Capsule （强肝胶囊, QGC） in treating non-alcoholic fatty liver disease （NAFLD）, using potyene phosphatidylcholine capsule （PPC） as a reference. Metheds： Eighty-eight patients with NAFLD were randomly assigned to two groups, 45 in the treatment group treated with QGC and 43 in the control group treated with PPC. The course of treatment tasted for 6 months. Changes in liver function, blood lipids, and iconographic indexes before and after treatment were observed, and clinical efficacy was evaluated. Results： In the treatment group, alanine aminotransferase （ALT） was towered significantly from 56.02 ± 32.59 lUlL before treatment to 38.27 ± 22.68 IU/L after treatment, and CT liver/spleen ratio significantly increased from 0.69± 0.18 to 0.91 ± 0.25, showing statistical significance （P〈0.05）; in contrast, the corresponding changes of the two indexes in the control group were 56.56 ±26.33 IU/L to 49.67 ± 26.22 IU/L, and 0.66± 0.20 to 0.75 ± 0.24, respectively, the pre-post treatment difference showing insignificant difference （P〉0.05）. No severe adverse reactions occurred during the whole treatment course. Conclusion： QGC is an effective and safe remedy for the treatment of NAFLD.
Objective： To analyze the proteomic characteristics of Gan （肝））--ssttaaggnnaannccyy syndrome （（GGSSSS）） by seeking the differential protein in blood and tissues of GSS model rats.Methods： GSS model rats were established by chronic restraint stress,keeping rats in restrain chamber for 6 h every day for 21 successive days.Their blood and liver samples were collected at the end of experiment for differential protein detection with methods of isoelectrofocusing and polyacrylamide SDS-PAGE,silver staining,and scanning.The gel images were analyzed with Imagemaster 2D Elite software,and the excavated differential protein spots were identified with matrix assistant laser resolving TOF mass spectrometry,Western blot,ELISA,and RT-PCR,respectively.Results： A method for isolating the protein in blood serum and tissues by two-dimensional gel electrophoresis was established and optimized.Six serum proteins and three liver proteins that differentially expressed were identified.The down-regulated differential proteins in serum of GSS model rats were serum albumin precursor,beta 1 globin,antibody against muscle acetylcholine receptor,Ig lambda-2 C region,and transthyretin （TTR）,and those in liver tissue were aryl sulfotransferase,enoyl-CoA hydratase,and TTR.TTR down-regulation was found in both serum and liver.Preliminary biological information analysis showed that these differential proteins involved in immune,neuroendocrine,nutrition,and substance metabolism.Conclusion： Proteomic analysis of differential proteins showed that TTR,aryl sulfotransferase,and enoyl-CoA hydratase expressions are downregulated in the GSS model rats,suggesting that the susceptibility of cancer could be enhanced by chronic stress.
目的:建立并优化血清蛋白质组二维凝胶电脉(Two-dimensional gel electrophoresis,2-DE)分离技术,并将其运用于中医证型研究。方法:在常规蛋白质组2-DE分离技术的基础上对血清样品处理、胶条选择、上样量和聚焦参数等加以优化并摸索新的方法。结果:建立了稳定的血清蛋白质组2-DE分离技术,运用于肝郁证的研究并取得理想结果。结论:方法具有稳定性、可重复性,为进一步研究血清相关蛋白质组及中医证型奠定基础。
