Objective To investigate whether there is neogenesis of myelin sheath and neuron after transplantation of Schwann cells into cerebral hemorrhage lesion. Methods Schwann cells were expanded, labeled with BrdU in vitro and transplanted into rat cerebral hemorrhage with blood extracted from femoral artery and then injected into the basal nuclei. Double immunohistochemistry staining and electron microscopy were used to detect the expression of BrdU/MBP and BrdU/GAP-43 and remyelination. Results BrdU/MBP double positive cells could be seen at 1 week up to 16 weeks after transplantation of Schwann cells. Thin remyelination was observed under electron microscope. GAP-43 positive cells appeared after 12 weeks and were found more in Hippocamp. Conclusions Grafted Schwann cells participate in remyelination and promoter nerve restore in rat cerebral hemorrhage.
Background Various tissue engineering strategies have been developed to facilitate axonal regeneration after spinal cord injury. This study aimed to investigate whether neural stem cells （NSCs） could survive in poly（L-lactic-co-glycolic acid） （PLGA） scaffolds and, when cografted with Schwann cells （SCs）, could be induced to differentiate towards neurons which form synaptic connection and eventually facilitate axonal regeneration and myelination and motor function. Methods NSCs and SCs which were seeded within the directional PLGA scaffolds were implanted in hemisected adult rat spinal cord. Control rats were similarly injured and implanted of scaffolds with or without NSCs. Survival, migration, differentiation, synaptic formation of NSCs, axonal regeneration and myelination and motor function were analyzed. Student＇s t test was used to determine differences in surviving percentage of NSCs. One-way analysis of variance （ANOVA） was used to determine the differences in the number of axons myelinated in the scaffolds, the mean latency and amplitude of cortical motor evoked potentials （CMEPs） and Basso, Beattie ＆ Bresnahan locomotor rating scale （BBB） score. The X2 test was used to determine the differences in recovery percentage of CMEPs. Results NSCs survived, but the majority migrated into adjacent host cord and died mostly. Survival rate of NSCs with SCs was higher than that of NSCs without SCs （（1.7831±0.0402）% vs. （1.4911±0.0313）%, P 〈0.001）. Cografted with SCs, NSCs were induced to differentiate towards neurons and might form synaptic connection. The mean number of myelinated axons in PLGA＋NSCs＋SCs group was more than that in PLGA＋NSCs group and in PLGA group （（110.25±30.46） vs. （18.25±3.30） and （11.25±5.54）, P 〈0.01）. The percentage of CMEPs recovery in PLGA＋NSCs＋SCs group was higher than in the other groups （84.8% vs, 50.0% and 37.5%, P 〈0.05）. The amplitude of CMEPs in PLGA＋NSCs＋SCs group was higher than in the other gro
