The Tiefosi granitic pluton is located 5 km northwest of Xinyang City, northern Dabie Orogen, and was emplaced in the Proterozoic Qinling Group. SHRIMP zircon U-Pb dating suggests its crystallization at 436 ± 11 Ma. It is composed of monzogranite and syenogranite containing some amounts of muscovite and few mafic minerals. The rocks are characterized by high and restricted SiO2 content, low FeO, Fe2O3 and MgO contents, high K2O/Na2O ratio, and display high-K calc-alkaline and peraluminous (ACNK>1.1) characteristics. They are generally enriched in large ion lithophile elements (LILE) and depleted in high field strength elements (HFSE). They can be divided into three groups in light of rare earth elements (REE) and trace elements. Group I is moderate in ΣREE and characterized by the absence of Eu anomaly, high (La/Yb)N ratio, and moderate Rb/Sr and Rb/Ba ratios. Group II has moderately negative Eu anomaly, low (La/Yb)N ratio and high ΣREE contents, Rb/Sr and Rb/Ba ratios. Group III displays positive Eu anomaly, moderate (La/Yb)N ratio, and low ΣREE, Rb/Sr and Rb/Ba ratios. The calculated ? Nd(440Ma) values of the rocks vary from ?8.8 to ?9.9 and Nd depleted mantle model ages are about 2.0 Ga, which resemble those of the paragneisses from the Qinling Group. The results indicate that the Tiefosi granite is crust-derived, syn-collisional S-type granite. Generation of Group I was related to low degree melting of the Qinling Group, while Group II was formed by fractionational crystallization of plagioclase from Group I magmas, and Group III resulted possibly from magma mingling with plagioclase cumulates. The Tiefosi granite was formed within crustal level related to the collision between the North China and South China blocks in the Early Paleozoic time.